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. 2021 Dec 20;10(12):2020. doi: 10.3390/antiox10122020

Figure 5.

Figure 5

Near total deletion of CCN2 expression resulted in reduced oxidative stress response 3 days after IRI. (A,B) Representative micrographs of mouse renal cortex stained with 4-hydroxynonenal (4-HNE; A) and nitrotyrosine (B). Quantification of 4-HNE (A) and nitrotyrosine (B) staining showed that an IRI-induced increase of lipid peroxidation and protein nitrosylation, respectively, decreased in CCN2 KO mice compared with WT mice. (C,D) qPCR analysis showed that increased mRNA expression of HMOX-1 (C) and NQO1 (D) in IRI kidneys significantly reduced in CCN2 KO mice compared with WT mice. Data are expressed as the mean ± SEM (n = 4–5 for WT sham; n = 6–7 for WT IRI; n = 4–5 for KO sham; n = 8–10 for KO IRI). TBP was used as an internal control. * p < 0.05, ** p < 0.01, and *** p < 0.001. Bar = 100 µm.