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. 2021 Dec 8;9(12):1859. doi: 10.3390/biomedicines9121859

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of isolation methods on the inhibitory concentration of oligomycin, and optimum concentrations of uncoupler FCCP and digitonin. (A) Adenosine triphosphate (ATP)-synthase inhibitor oligomycin. (B) Uncoupler FCCP. (C) Mild detergent digitonin for plasma membrane permeabilization. (D) Representative trace of the viability module of the SUIT protocol. DC (black) and PA (red). Oxygen flow per cell I_O2 [amol·s⁻¹·x⁻¹]. One-way ANOVA with multiple comparison test (Tukey), p values are shown above their respective pairwise comparison bar.