Figure 8.

PCNA immunoreactivity in intact skin. (a) A representative image showing PCNA immunoreactivity in a transverse section of an intact forelimb. (b) Enlargement of the skin in the area indicated by the bracket in (a). Almost all the cell nuclei along the basal layer (BL) showed immunoreactivity (light brown). Nuclei in the tissue were counterstained purple with hematoxylin. (c) Control. A section next to the one shown in (a) was stained as in (a), but without primary antibody. (d) Enlargement of the skin in the area indicated by the bracket in (c). PCL: pigment cell layer; SC: stratum corneum; TL: transitional layer. Scale bars: 300 μm (a,c); 100 μm (b,d).