Figure 12.

Wound surface on which the wound epidermis extends. (a) Representative image showing the wound surface under an extending epidermal tongue (EET) at 18 h after operation. Asterisk: leading end. SC: stratum corneum. Line: position of the wound margin. The EET lay over either the fibrin-like membrane or the epimysium tissue with a space containing white blood cells. A part of the EET is enlarged in inset (b). Blue: DAPI stain of nuclei. Dotted line in green: the innermost margin of the EET. Dotted line in magenta: fibrin-like membrane. Nuclei of white blood cells were recognized on the fibrin-like membrane. A break of the fibrin membrane near the leading end of the EET, as indicated by an arrow in (a), is enlarged in inset c. Scale bars: 50 μm (a); 20 μm (b,c).