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Telomerase activity in wild-type and DNA-PKcs−/− MEFs. S-100 extracts were prepared from wild-type (C3, C5, and B5), DNA-PKcs+/− (B3), and DNA-PKcs−/− (C2, C9, B4, and B6) MEFs and assayed for telomerase activity. Extracts were pretreated (+) or not (−) with RNase. The protein concentrations used are indicated. Arrow, internal control (IC) for PCR efficiency. The same letter indicates littermate embryos.
