TABLE 3.
Measurement by Flow-FISH of telomere length in splenocytes and BM cells obtained from DNA-PKcs+/+, DNA-PKcs+/−, and DNA-PKcs−/− mice
| Mouse (sexc) | Litter | Genotype | Telomere lengtha for:
|
|
|---|---|---|---|---|
| Splenocytes | BM cells | |||
| D33 (F) | 1 | −/− | 3.55 | |
| D34 (F) | +/+ | 2.83 | ||
| D36 (M) | 2 | −/− | 3.20 | |
| D44 (F) | +/− | 2.82 | ||
| D49 (F) | 3 | −/− | 3.08 | |
| D54 (F) | +/− | 2.29 | ||
| DA38 (F) | 4 | −/− | 3.49 | 3.56 |
| DA39 (F) | −/− | 3.43 | 3.33 | |
| DA40 (F) | +/+ | 3.34 | 3.29 | |
| DA45 (M) | 5 | +/+ | 3.69 | 3.08 |
| DA46 (M) | +/+ | 3.56 | 3.47 | |
| DA47 (F) | −/− | 3.73 | 4.01 | |
| DA48 (F) | −/− | 3.93 | 3.29 | |
| DA50 (M) | 6 | +/− | 3.77 | 3.14 |
| DA51 (M) | +/− | 4.07 | 3.41 | |
| DA52 (M) | −/− | 4.11 | 3.48 | |
| DA58 (M) | 7b | −/− | 3.15 | 3.59 |
| DA59 (M) | +/− | 3.65 | 3.05 | |
| DA60 (M) | +/+ | 3.75 | 3.74 | |
| DA61 (F) | −/− | 3.82 | 3.51 | |
| DA62 (F) | −/− | 3.98 | 3.44 | |
| DA64 (F) | +/+ | 4.22 | 3.55 | |
a
Telomere length is expressed in arbitrary fluorescence units which have been normalized against a calibration curve on R and S cells (see Materials and Methods).
b
The animals of litter 7 were analyzed in two different experiments.
c
M, male; F, female.