Skip to main content
. 2021 Dec 16;10(12):1343. doi: 10.3390/biology10121343

Figure 2.

Figure 2

Strategy of the construction of food-grade expressing plasmids. (a) Construction of a plasmid pMA5-alrA expressing alanine racemase. Kanamycin and bleomycin were replaced by the enzyme alanine racemase alrA derived from B. subtilis 168 to construct expression plasmid pMA5-alrA based on plasmid pMA5. (b,c) The β-galactosidase and arabinose isomerase genes from E. coli K-12 were inserted between the multiple cloning sites NdeI and MluI of the constructed plasmid pMA5-alrA to separately construct plasmids pMA5-alrA-lacZ and pMA5-alrA-araA. (d) The arabinose isomerase gene was inserted between the KpnI and HindIII sites from the constructed plasmids pMA5-alrA-lacZ to construct the co-expressing plasmid pMA5-alrA-lacZ-araA.