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. 2021 Nov 25;10(12):1883. doi: 10.3390/antiox10121883

Figure 6.

Figure 6

Pretreatment with antioxidant NAC rescued both intracellular and mitochondrial ROS accumulation and apoptosis induced by pardaxin in PA-1 and SKOV3 cells. (A) Flow cytometric analyses of cells treated with or without NAC and/or pardaxin using Annexin V and PI staining; (B) quantitation of Annexin V/PI detection signals; (C) flow cytometric analyses of cells treated with or without NAC and/or pardaxin using CellROX™ Green fluorogenic dye; (D) quantitation of the CellROX™ Green detection signals; (E) flow cytometric analyses of cells treated with or without NAC and/or pardaxin using DCF dye; (F) quantitation of the DCF detection signals; (G) flow cytometric analyses of cells treated with or without NAC and/or pardaxin using MitoSOX™ Red fluorogenic dye; (H) quantitation of the MitoSOX™ Red detection signals; (I) flow cytometric analyses of cells treated with or without NAC and/or pardaxin using AO fluorogenic dye; (J) quantitation of the AO detection signals. Each bar represents the result of mean ± SE determined from three independent experiments and the analyses were conducted using ANOVA method. ** p < 0.01 show statistical significance compared with the control group (without NAC and pardaxin), and # p < 0.01 relative to the experimental group with 5 μM of pardaxin alone.