Figure 1.

Treatments’ effect on neutrophil recruitment to the lesion site in zebrafish (Tg(mpxGFP)ⁱ¹¹⁴) larvae induced by tail transection. Data from at least five independent samples of five random animals each. (A) Illustration of the experimental protocol of the neutrophil migration study. (B) Illustrative image of a normal Tg(mpxGFP)i114 (control) and a larva with transection of the tail (control cut). Detailed images of the local site of the transection after exposure to treatments during 4 hpi. The scale bar represents 125 μm. (C) Number of neutrophils migrated to the tail at 4 hpi. Values were normalised according to the control group. Data are expressed as mean ± SD and statistical analysis was performed using a one-way ANOVA followed by Tukey’s multiple comparison test. Different letters represent statistical differences among treatment groups (p < 0.05).