Figure 12.

(A) Dermal fibroblast scratch wound closure over time after induction of chronic inflammatory conditions (TNF-α/IFN-γ) and treatment with wIRA or heating w/o wIRA. (B) Evaluation of scratch wound healing in [%]. (C) Gene expression profiles of these fibroblasts were assessed for pro-inflammatory cytokine gene expression after 1 h (IL1A, IL6) and 6 h (CXCL8) as well as stress response gene together with heat shock protein gene transcript levels. Growth factor gene expression was appraised after 24 h and collagen gene expression was evaluated after 48 h. Asterisks [*] indicate significant differences in scratch wound healing or transcription level compared to 1 h (* p < 0.05, *** p < 0.001). Hashtags [#] designate significant differences of scratch wound healing or transcription levels at the respective time point compared to the control under optimal conditions (# p < 0.05, ## p < 0.01, ### p < 0.001) while the paragraph character [§] shows significant differences between untreated and treated samples under non-optimal conditions at the respective time point (§ p < 0.05, §§ p < 0.001, §§§ p < 0.001). The plus sign [+] specifies significant differences between wIRA-treated samples and those receiving heating w/o wIRA under non-optimal conditions at the respective time point (+ p < 0.05, +++ p < 0.01).