Figure 4.

Sorting of the RNA-seq data. (A) A heat map was populated with 3586 genes that were sorted based on the differential expression among the samples, as explained in Figure 3. (B) The Pearson’s correlation based on the shortlist of 3586 genes showed how the samples analyzed in this study correlate expression-wise. Two main clusters of samples are visible: one that is stem cell-enriched (X1, X2 samples) and one that is non-stem cell-enriched (Xin, RoB, Irr d3, Irr d6, SmB(RNAi), blastema samples). Within the non-stem cell-enriched samples, the blastema samples clustered together in a sub-group (second most relevant hierarchical division). (C–E) The 3586 genes were grouped according to their molecular function (C). A lower hierarchical level of molecular functions was showed by the different classes of genes with binding activity (D). The pie chart showed how the 111 genes with nucleic acid binding activity distribute in the two main classes of nucleic acid binding: DNA- and RNA-binding (E).