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. 2021 Dec 17;10(12):1551. doi: 10.3390/antibiotics10121551

Figure 4.

Figure 4

Relative ompK35 and ompK36 expression levels in K. pneumoniae. RT-qPCR assay of ompK35 and ompK36 expression was performed in K. pneumoniae cluster A (a,c), a singleton isolate KP162 (a,c), cluster B (b,d). The relative number of ompK35 and ompK36 transcripts of K. pneumoniae isolates was normalized to 16S rRNA expression and calculated using the 2−ΔΔct method compared to the expression of imipenem-susceptible K. pneumoniae isolates KP203 and KP221. p-values were calculated using unpaired t-test (*, p-value < 0.05; **, p-value < 0.01; ***, p-value < 0.001 and ns, non-significant). Killing effect of imipenem against K. pneumoniae cluster A and a singleton (e), and cluster B (f). Mean of bacterial viable cells at the treatment with 1 × MIC of imipenem was plotted at 0, 2, 4, 6, and 24 h of incubation. All experiments were performed in triplicate and the detection limit of the viable cells was 102 CFU/mL (dashed lines).