Table 2.

Thermodynamic parameters of inhibitor 9 measured in this and a previous study by Rechlin et al. [4].

Inhibitor	KD [µM]	ΔG° [kJ/mol]	ΔH° [kJ/mol]	–TΔS° [kJ/mol]	Titration Mode
9 (from this study)	0.42 ± 0.08	−36.4 ± 0.5	−53.3 ± 0.5	16.9 ± 0.7	direct
9 (derived from [4])	0.05 ± 0.01	−41.5 ± 0.4	−54.0 ± 1.0	12.5 ± 1.1	direct

The thermodynamic data were determined by ITC in HEPES buffer pH 8.0. The data are not corrected for the overlaying protonation effect.