Table 2.
Composition of gastric organoid growth medium.
| Reagents | Final Concentration | Manufacturer |
|---|---|---|
| Advanced DMEM/F12 | Thermo Fisher Scientific Waltham, MA, USA |
|
| Penicillin/Streptomycin | 1% | Thermo Fisher Scientific Waltham, MA, USA |
| Hepes | 10 mM | Thermo Fisher Scientific Waltham, MA, USA |
| Glutamax | 1× | Thermo Fisher Scientific Waltham, MA, USA |
| B27 | 1× | Thermo Fisher Scientific Waltham, MA, USA |
| N2 | 1× | Thermo Fisher Scientific Waltham, MA, USA |
| Nicotinamide | 10 mM | Sigma-Aldrich St. Louis, MI, USA |
| N-Acetyl cysteine | 1 mM | Sigma-Aldrich St. Louis, MI, USA |
| Y-27632 | 10 µM | Sigma-Aldrich St. Louis, MI, USA |
| Noggin | 100 ng/mL | Peprotech Cranbury, NJ, USA |
| FGF-10 | 200 ng/mL | Peprotech Cranbury, NJ, USA |
| EGF | 50 ng/mL | Peprotech Cranbury, NJ, USA |
| Gastrin 1 | 1 nM | TOCRIS Bioscience |
| Wnt Conditioned Medium | 50% | |
| R-Spondin Conditioned Medium | 10% |