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. 2021 Nov 29;9(12):1793. doi: 10.3390/biomedicines9121793

Figure 1.

Figure 1

Effects of the administration of AX and injection of ISO on the content of Troponin I, ALT, Myoglobin, AST, and LDG in rat heart tissue. Protein samples were extracted and subjected to Western blotting. The immunodetection of GAPDH was used as a loading control. (a)—Immunostaining with Troponin I, ALT, Myoglobin antibodies and GAPDH (upper part); quantification of immunostaining by computer-assisted densitometry presents as a ratio of proteins to GAPDH (low part), (b)—Immunostaining with AST and LDG antibodies and GAPDH (upper part); quantification of immunostaining by computer-assisted densitometry presented as a ratio of proteins to GAPDH (low part). Bar graphs represent the levels of appropriate proteins; the data are presented as the means ± SD of three independent experiments. * p < 0.05 significant difference in the protein level in comparison with the control (group 1). # p < 0.05 compared to RHM isolated from ISO-injected rats (group 3). The statistical significance of the differences between the pairs of mean values was evaluated using the Student-Newman-Keul test.