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. 2021 Nov 29;9(12):1793. doi: 10.3390/biomedicines9121793

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The effect of chronic treatment of AX on the activity of supercomplexes (SCs) in rat heart mitochondria in heart failure. (a)—the first dimension after separation of the mitochondrial suspension by BNE (non-denaturing condition) Coomassie staining, Complex V and I activity (upper part); diagram reflecting the change in the activity of Complexes V and I (low part). Optic density of Coomassie bands was used as a loading control; (b)—the second dimension (Laemmli system under denaturing conditions) after cutting strips from the gel after the first measurement; (c)—the analysis of the amino acid sequence (protein coverage). Data are presented as means ±SDs of four independent experiments. * p < 0.05 indicates a significant difference in the protein level relative to the control (group 1). # p < 0.05 compared to RHM isolated from ISO-injected rats (group 3). The statistical significance of the differences between the pairs of the mean values was evaluated using an ANOVA type 2 (Student–Newman–Keuls) test.