Figure 5.

Effects of the administration of AX on the content of ATPB, ATP5F1, ATP5G, pGSK3β and GSK3β in rat heart mitochondria in heart failure. (a)—immunostaining with ATPB, ATP5F1, ATP5G, and Tom20 antibodies (upper part); low part—quantification of immunostaining by computer-assisted densitometry presented as a ratio of proteins to Tom20; (b)—immunostaining with pGSK3β, GSK3β, and Tom20 antibodies (upper part); low part—quantification of immunostaining by computer-assisted densitometry presented as a ratio of proteins to Tom20. Bar graphs represent the levels of appropriate proteins; the data are presented as the means ± SD of three independent experiments. * p < 0.05 significant difference in the protein level in comparison with the control (group 1). # p < 0.05 compared to RHM isolated from ISO-injected rats (group 3). The statistical significance of the differences between the pairs of mean values was evaluated using the Student-Newman-Keul test.