FIG. 5.

T antigen targets TFIID to inhibit DA complex assembly. (A) p53-T complex (p53/T) prevents TFIID from binding to the promoter. Mg-EMSA reactions were carried out with TFIID complex, p53, and p53-T complex (as indicated above each lane) using the 5RGCE4T probe. (B) A preassembled complex containing DNA and TFIID is partially resistant to p53-T complex inhibition. TFIID was incubated with the probe for 30 min at 30°C in either the presence or absence of increasing amounts of p53-T complex. p53-T complex was then added to the reaction mixtures in lanes 5 and 6 and was incubated for an additional 30 min prior to the Mg-EMSA. (C) A preassembled complex containing DNA and TBP is not resistant to p53-T complex inhibition. Experiments were performed as for panel A but with 10 ng of TBP instead of TFIID. All preincubation Mg-EMSA reactions were carried out for 60 min.