Table 1.
List of antibodies used for phenotypic characterisation of circulating tumour cells on the ImageStream®X platform.
| Primary | Secondary | Fluorophore | Dilution | Reference | Brand | IS Ch |
|---|---|---|---|---|---|---|
| * Mouse IgG2a anti-cytokeratin 7/8 | None | FITC | 1/100 | 130-060-301 | Miltenyi, Germany | 2 |
| Rabbit monoclonal [K21-F] to BRAF (mutatedV600E) | None | FITC | 1/200 | ab175637 | Abcam, UK | 2 |
| Mouse monoclonal [CAM5.2] anti-human cytokeratin (7/8) | None | PE-CF594 | 1/100 | 563615 | BD, USA | 4 |
| None | None | 7-AAD | 1/100 | 00-6993-50 | Invitrogen, USA | 5 |
| Rabbit polyclonal to PD-L1 | Goat anti-rabbit IgG | DyLight 405 | 1/100 1/100 |
PA5-28115 35551 |
Thermo Fisher, USA | 7 |
| Mouse monoclonal [2D1] anti-human CD45 APC CY7 | None | APC-Cy7 | 1/100 | 557833 | BD, USA | 12 |
* Anti-CK-FITC was only used for control experiments to ensure epithelial origin, but for CTC characterisation, anti-CK-PE-CF594 antibody was used instead; abbreviation “IS Ch” refers to “ImageStream®X channel”.