Figure 10.

Effect of butyrate on ARDS induced by SEB. On day −1, 0, and 1, mice received 200 mg/kg of butyrate (BUT) or VEH through oral gavage, while SEB at a dose of 50 μg/mouse was given intranasally on day 0. Mice were euthanized on day 2 for various studies (48 h after SEB exposure). (A) Representative histopathological images and scores.Images were generated by using Keyence microscope with 40x magnification planAPO NA0.95 and H&E staining of excised lung tissue from Naïve, SEB + VEH and SEB + BUT groups of mice. (B,C) Use of plethysmography to measure the clinical function of the lung, including (B) specific airway resistance and (C) specific airway conductance. Five mice in each group for histopathology and four mice per group were used for the experiment on plethysmography, and the results were confirmed in three independent experiments. One-way ANOVA with post hoc Tukey’s test was used to analyze for significance and was indicated as follows: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p < 0.0001.