Table 1.

Sequences of DNA blocks used for CRISPR/Cas9 cloning.

Block	Sense	Sequence (5′ → 3′)
miR-scamble	Forward	5′-CCGGTACACCATGTTGCCAGTCTCTAGGTGGGCGTATAGACGTGTTACACTGTGAAGCCACAGATGTGTAACACGTCTATACGCCCATGGCGTCTGGCCCAACCACACTTTTTG-3′
	Reverse	5′-AATTCAAAAAGTGTGGTTGGGCCAGACGCCATGGGCGTATAGACGTGTTACACATCTGTGGCTTCACAGTGTAACACGTCTATACGCCCACCTAGAGACTGGCAACATGGTGTA-3′
miR-210	Forward	5′-CCGGTACACCATGTTGCCAGTCTCTAGGAGCCCCTGCCCACCGCACACTGTGTGAAGCCACAGATCTGTGCGTGTGACAGCGGCTGATGGCGTCTGGCCCAACCACACTTTTTG-3′
	Reverse	5′-AATTCAAAAAGTGTGGTTGGGCCAGACGCCATCAGCCGCTGTCACACGCACAGATCTGTGGCTTCACACAGTGTGCGGTGGGCAGGGGCTCCTAGAGACTGGCAACATGGTGTA-3′
Anti-miR-control	Forward	5′-CCGGTAGAGCTCCCTTCAATCCAAGTGAAGAGCTCCCTTCAATCCAACGCGTAGAGCTCCCTTCAATCCAAATCGAGAGCTCCCTTCAATCCAACGCGTAGAGCTCCCTTCAATCCAAGTGAAGAGCTCCCTTCAATCCAACGCGTAGAGCTCCCTTCAATCCAAATCGAGAGCTCCCTTCAATCCAATTTTTG-3′
	Reverse	5′-AATTCAAAAATTGGATTGAAGGGAGCTCTCGATTTGGATTGAAGGGAGCTCTACGCGTTGGATTGAAGGGAGCTCTTCACTTGGATTGAAGGGAGCTCTACGCGTTGGATTGAAGGGAGCTCTCGATTTGGATTGAAGGGAGCTCTACGCGTTGGATTGAAGGGAGCTCTTCACTTGGATTGAAGGGAGCTCTA-3′
Anti-miR-210-3p	Forward	5′-CCGGTTCAGCCGCTGTCACACGCACAGGTGATCAGCCGCTGTCACACGCACAGCGCGTTCAGCCGCTGTCACACGCACAGATCGTCAGCCGCTGTCACACGCACAGCGCGTTCAGCCGCTGTCACACGCACAGGTGATCAGCCGCTGTCACACGCACAGCGCGTTCAGCCGCTGTCACACGCACAGATCGTCAGCCGCTGTCACACGCACAGTTTTTG-3′
	Reverse	5′-AATTCAAAAACTGTGCGTGTGACAGCGGCTGACGATCTGTGCGTGTGACAGCGGCTGAACGCGCTGTGCGTGTGACAGCGGCTGATCACCTGTGCGTGTGACAGCGGCTGAACGCGCTGTGCGTGTGACAGCGGCTGACGATCTGTGCGTGTGACAGCGGCTGAACGCGCTGTGCGTGTGACAGCGGCTGATCACCTGTGCGTGTGACAGCGGCTGAA-3′
SgRNA AAVS1		5′-GGGGCCACTAGGGACAGGATTGG-3′