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. 2021 Nov 24;10(12):3286. doi: 10.3390/cells10123286

Figure 1.

Figure 1

Selective KD of α7 nAChRs in the hippocampus differentially regulates the expression of Table 1. gene during synaptogenesis and synaptic maturation stage (in vitro). (A) live cell phase contrast (i–iii), (B) tdTomato fluorescence (i–iii); images of untreated control (Ai,Bi), scrambled control (Aii,Bii) and α7 shRNA AAV (Aiii,Biii) AAV transduced hippocampal cultures on DIV 7 (n = 21 images, 7 independent samples each, representative images). Scale bar 20 μm. (C,D) Summary data, fold change gene expression in hippocampal cultures on DIV 10 (C) and DIV 20 (D), relative to untreated control, determined by qPCR (n = 6, three independent experiments each, triplicate replicates). α7 KD upregulated MEN1 expression initially and downregulated later in the synaptic maturation stage. Statistical significance (one-way ANOVA followed by Tukey’s multiple comparison test) **** p < 0.0001, ns p > 0.9999. See Supplementary Table S2.