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. 2021 Nov 24;10(12):3286. doi: 10.3390/cells10123286

Figure 2.

Figure 2

Reduced expression of Bungarotoxin labelled α7 nAChRs in α7 KD neurons (in vitro). (A) High-magnification (60×) confocal image of α7-nAChR shRNA AAV-transduced KD hippocampal neurons (Aiv–vi) compared to scrambled controls (Ai–iii) on DIV 10 (n = 30 images, six independent samples, representative image). tdTomato-positive cells (Aii,v) labelled with α-bungarotoxin (α7-nAChR) (Ai,iv) and merged (Aiii,vi), Aiv exhibits reduced expression of α7-nAChR in tdTomato-positive cells. Scale bars, (Ai–iii) 15 and (Aiv–vi) 15 μm. (B) Summary data, ICC characterization of α7-nAChR protein expression (normalized) in neuronal soma and neurites from scrambled shRNA and α7-KD hippocampal cultures compared to untreated controls (n ≥ 0 images, ≥6 independent samples, DIV 3, 7, 10, 14 and 20). Arrowheads indicate tdTomato-positive neurons. Statistical significance (one-way ANOVA followed by Tukey’s multiple comparison test) **** p < 0.0001, ns p > 0.9999. See Supplementary Table S3.