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. 2021 Nov 24;10(12):3286. doi: 10.3390/cells10123286

Figure 4.

Figure 4

PSD-95 puncta expression reduces in α7 KD neurons (in vitro). (A) High-magnification (60×) confocal image of a α7 KD hippocampal neuron (Av–viii) compared to scrambled controls (Ai,iv) on DIV 10 (n = 30 images, six independent samples, representative image). tdTomato-positive cells (Aii,vi) co-labelled with α-bungarotoxin (α7-nAChR) (Ai,v) and PSD-95 (postsynaptic protein) (Aiii,vii) and merged (Aiv,viii). (Avii) exhibits the attenuated expression of PSD-95 in α7 KD hippocampal neurons compared to the scrambled control (Aiii). Scale bars, (Ai–iv) 25 and (Av–viii) 20 μm. (B) Summary data, normalized PSD-95 expression in neuronal soma and neurites from scrambled shRNA and α7-KD hippocampal cultures compared to untreated controls (n ≥ 25 images, ≥6 independent samples, DIV 3, 7, 10, 14 and 20). Yellow arrowheads indicate tdTomato-positive neurons. Red asterisks indicate bungarotoxin-positive puncta. Green asterisks indicate PSD-95 puncta. Statistical significance (one-way ANOVA followed by Tukey’s multiple comparison test) **** p < 0.0001, ns p > 0.9999. See Supplementary Table S5.