Skip to main content
. 2021 Nov 24;10(12):3286. doi: 10.3390/cells10123286

Figure 6.

Figure 6

Downregulation of the MEN1 gene in α7 KD mouse hippocampus (in vivo). (A) Illustrative representation of experimental protocol for bilateral stereotaxic injections in CA1 coordinates. (B) Summary data, fold change gene expression in α7-KD hippocampal tissue relative to untreated control, determined by qPCR (n = 6, four independent experiments each, triplicate replicates). Downregulation of the MEN1 gene in α7-KD tissue. (C) IHC characterization of α7 KD hippocampal slices (Civ–vi) compared to scrambled controls (Ci–iii) 8 weeks post-surgery (n = 25 images, six independent samples, representative image). tdTomato-positive neurons (Ci,iv) labelled with α-bungarotoxin (α7-nAChR) (Cii,v) and merged (Ciii,vi), Av exhibits significantly reduced expression of α7-nAChR in tdTomato-positive hippocampal pyramidal neurons. Scale bars, (Ci–iii) 25 and (Civ–vi) 40 μm. Yellow dotted lines indicate tdTomato-positive pyramidal neuronal layers. White asterisks indicate bungarotoxin-positive puncta. Statistical significance (one-way ANOVA followed by Tukey’s multiple comparison test) **** p < 0.0001, * p < 0. 01, ns p > 0.9999. See Supplementary Table S7.