Figure 6.

EGCG recovers Ca²⁺ dysregulation in PSEN1 E280A ChLNs. After 7 days of transdifferentiation, WT PSEN1 and PSEN1 E280A ChLNs were left untreated or treated with EGCG in a regular culture medium for 4 days. (A–D) Time-lapse images (0, 10, 20, 30, 40, 50, and 60 s) of Ca²⁺ fluorescence in WT PSEN1 and PSEN1 E280A ChLNs on day 4 in response to ACh treatment. ACh was puffed into the culture at 0 s (arrow). Then, the Ca²⁺ fluorescence of the cells was monitored at the indicated times. Color contrast indicates fluorescence intensity: dark blue < light blue < green < yellow < red. (E) Normalized mean fluorescence signal (∆F/F) over time from the cells indicating temporal cytoplasmic Ca²⁺ elevation in response to ACh treatment. Data are presented as the mean ± SD. ***^{, ###, $$$} p < 0.001; *** represents differences compared to UNT PSEN1 E280A cells; ^### represents differences compared to UNT PSEN1 WT or E280A cells; ^$$$ represents differences compared to EGCG-treated PSEN1 E280A cells. The figures represent 1 out of 3 independent experiments.