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PCR detection of soybean allergens: Gly m Bd 28K using primer pairs 28K86f/28K86r (A, lanes 1–3) and 28K98f/28K98r (A, lanes 4–6), Gly m Bd 30K using primer pairs 30K122f/30K122r (B, lanes 1–3), 30K129f/30K129r (B, lanes 4–6), 30K118f/30K118r (C, lanes, 1–3). Samples: lanes 1–2, 4–5. soybean seeds flour, lanes 3, 6. water (negative control). M. Molecular weight marker (Qiagen GelPilot 100 bp ladder).
