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. 2021 Nov 26;11(12):481. doi: 10.3390/bios11120481

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Hybridization of asymmetric multiplex PCR (N4) products with the probes Lect101, epsps172, 28K86 and 30K122 on a biochip. (A) Hybridization image (a,b) designations of the columns for the probe arrangement; (B) The arrangement of the probes on the biochips; (C) Analysis of hybridization; the hybridization signal intensities estimated as signal to noise (S/N) ratio. (D) Agarose gel electrophoresis of asymmetric PCR products. Total DNA per hybridization is 1340 ng, the amount of fluorescent dye Cy3 is 18 pmol.