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Regulation of cytotoxic granules and intracellular production of cytokines by neutralizing 0.5 ug/mL of anti-IFN-γ (n = 5) and/or 10 ug/mL of anti-IL-12 (n = 5) antibodies in a 72 h-culture of HD PBMCs with 25% plasma from R patients. Percentages of CD8+ HLA-DR+ T cells producing intracellular (A) granzyme B, (B) perforin, (C) IFN-γ (D) TNF-α (E) and IL-10 determined by ELISA. * p < 0.05; *** p < 0.001.
