Figure 6.

(A) Two-photon imaging of local changes in the dendritic structure before, during, and after MCAO. In the left panel, intact dendritic structures were observed; in the middle panel, extensive dendritic blebbing was observed; in the right panel, a significant recovery of dendritic structures after reperfusion was observed. Modified by Li and Murphy 2008, Copyright (2008) Society for Neuroscience. (B) Time-lapse imaging of apical dendrites showed the retraction of a dendritic spine. Modified by Brown et al., 2007, Copyright (2007) Society for Neuroscience. (C) Time-lapse images of maximum intensity z-projections (from 20 to 60 μm) before (left) and after (right) the laser-induced ischemic hemorrhage. The figures shown in green are the GFP-labeled neurons in a GFP-M mouse, and in red are the vascular networks labeled with Texas-red dextran dye. The tip of the yellow lightning symbol represents the laser irradiation point. The first image was acquired just before the laser irradiation. Scale bar, 20 μm. Modified from Allegra Mascaro et al., 2010. (D) Image sequences of cortical activation as assessed by calcium imaging during pulling of the handle by the contralateral forelimb of CTRL (top), STROKE (bottom) Thy1-GCaMP6f mice in the M-Platform. A small area located in the motor-sensory region reproducibly lit up in CTRL mice, while a large area covering most of the cortical surface of the injured hemisphere was activated in STROKE mice 1 month after stroke. A–P, anterior posterior, M-L, medio-lateral, M1, primary motor area, V1, primary visual area, S1, primary sensory area, Rs, Retro splenial area, BF, barrel field. The black dashed lines define the lesion borders. The black dot indicates bregma. Scale bar 1 mm. Modified from Allegra Mascaro et al., 2019.