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Tunneling nanotubes formation in bladder cancer cell lines. RT4, RT112, 5637, HT1376, UMUC-3 and J82 cells were plated at a density of 1 × 104 cells/well on CellCarrier Ultra 96-well microplates. After 24 h, live cell images were acquired using Operetta CLS™ equipped with 63× immersion objective in brightfield (left panels) and Digital Phase Contrast (DPC) (right panels). Scale bar: 50 µm. Magnifications show TNTs.
