Figure 10.

RalGPS2 interacts with Akt, PDK1, TNFαIP2 and p53 P in 5637 and HEK293 cells. (A) 5637 and (B) HEK293 cells were plated in 100 mm dishes and the day after were first serum-starved for 18 h and then stimulated with 10% FBS for 15 min (S) or left unstimulated (NS). Subsequently, immunoprecipitation (IP) with anti-RalGPS2 antibodies was performed, as indicated in each panel. Immunoprecipitates were assessed for the presence of total Akt (Akt tot), phospho-Akt (Akt P), PDK1, TNFαIP2 and phospho-p53 (p53 P). Three independent experiments were performed.