Figure 12.

RalGPS2 interacts with RalA, LST1 and Sec5 in HEK293 cells. HEK293 cells were plated in 100 mm dishes, and the day after, were first serum-starved for 18 h and then stimulated with 10% FBS for 15 min (S) or left unstimulated (NS). Subsequently, immunoprecipitation (IP) with (A) anti-RalGPS2, (B) anti-LST1 or (C) anti-Sec5 antibodies were performed, as indicated in each panel. Immunoprecipitates were probed for Sec5, LST1, RalA and RalGPS2 presence. tLST1: LST1 trimerous; mLST1: LST1 monomer.