Table 6. Domestic and sentinel animals with evidence of MAYV infection.

Animal Type	Total Positive	Number Testeda	% Pos	Test Method	Notes	Citation
Domestic Equids	16	213	7.5	ELISA with confirmatory plaque reduction NT	Forty-eight horses had antibodies to MAYV by ELISA. Sixteen of 48 (33%) were considered positive by plaque reduction NT90 for MAYV with titers 1:10 (n = 12), 1:20 (n = 3) and 1:40 (n = 1).	[78]
	4	753	0.5	HI	Forty reactions overall. Four of 40 reactions were monotypic while 36 of 40 were heterotypic.	[67]
	11	102	10.8	HI	Not clear if the 11 reactions are monotypic or heterotypic.	[61]
	10	748	1.5	Plaque reduction NT	Forty-four horses had neutralizing antibody (titer ≥ 10) against MAYV, but only ten met the diagnostic criteria of 4-fold greater plaque reduction NT90 titer than the three other viruses (VEEV, EEEV, WEEV). Positive samples had titers of 1:20 (n = 6) and 1:40 (n = 4)	[90]
Domestic Cattle/Buffalo	5	1103	0.5	HI	Positive reactions were considered any reaction with a titer equal to or greater than 1:20. Fourteen reactions overall, and five of 14 reactions were monotypic.	[66]
Domestic Dog	2	7	28.6	HI	N/A	[57]
Sentinel Hamster	1	N/A	N/A	RT-PCR		[86]
Sentinel Monkeys	2	13	15.4	HI	N/A	[102]

MAYV: Mayaro virus; HI: hemagglutination inhibition; ELISA: enzyme-linked immunosorbent assay; RT-PCR: reverse transcription polymerase chain reaction; NT: neutralization test; VEEV: Venezuelan equine encephalitis virus; EEEV: Eastern equine encephalitis virus; WEEV: Western equine encephalitis virus

^a Denominators presented in this table reflect only studies that reported MAYV positivity. Complete data (including MAYV-negative samples) are reflected in the seroprevalence meta-analysis and the S3 Table.