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Effect of miR-34a overexpression on EMT-related transcriptional factors in A549 and H1299 cells. These cells were transfected with NC (20 pmol/well) or miR-34a mimic (20 pmol/well) for 24 h. After 24 (A) or 72 h (B), the mRNA expression levels of SNAIL, SLUG, zinc finger E-box binding protein (ZEB)-1, and TWIST were measured by real-time PCR. Each value represents the mean ± S.E.M. (n = 3). ** p < 0.01, significantly different from NC.
