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Polyacrylamide gel electrophoresis (PAGE) of LOXL2s. (A) SDS-PAGE under denaturing conditions in the presence and absence of reductant (β-mercaptoethanol). (B) Native-PAGE. AAQ: R315A/R316A/K317Q mutant form of LOXL2; WT: wild type; K317R-LOXL2 was isolated as a mixture of fl-LOXL2 and Δ1-2SRCR-LOXL2; Δ1-2SRCR-LOXL2 was isolated from the mixture by FPLC; r-P4H: recombinant Bacillus anthracis prolyl-4-hydroxylase as a molecular standard (homodimer, 49.2 kDa) [22].
