Table 1.
Comparison of spontaneously beating and non-beating iCell cardiomyocytes.
| iCell Cardiomyocytes | |||
|---|---|---|---|
| Spontaneous Beat Rate (n Cells) |
=0 (8) |
>0 (47) |
|
| HCN4 | % pos. cells ø CT ± SD |
100 27.8 ± 0.6 |
100 28.0 ± 0.2 |
| CACNA1G | % pos. cells ø CT ± SD |
25 30.1 ± 0.1 |
43 29.9 ± 0.4 |
| CACNA1D | % pos. cells ø CT ± SD |
13
29.5 ± 0.03 |
32
31.7 ± 0.4 *** |
| KCNA5 | % pos. cells ø CT ± SD |
38 32.6 ± 0.4 |
28 31.9 ± 0.4 |
| KCNJ4 | % pos. cells ø CT ± SD |
75 30.8 ± 0.6 |
64 31.0 ± 0.2 |
| SCN5A | % pos. cells ø CT ± SD |
100 27.9 ± 0.4 |
100 27.4 ± 0.2 |
| KCNJ2 | % pos. cells ø CT ± SD |
25 31.6 ± 1.2 |
32 31.8 ± 0.3 |
| CACNA1C | % pos. cells ø CT ± SD |
100 27.7 ± 0.9 |
98 27.4 ± 0.3 |
| KCNH2 | % pos. cells ø CT ± SD |
100 27.9 ± 0.7 |
98 27.2 ± 0.2 |
| ø Capacitance [pF] ± SD | 14.68 ± 1.80 | 27.25 ± 2.28 *** | |
| ø Fast inward current amplitude [nA] ± SD | −10.36 ± 2.33 | −17.30 ± 1.87 * | |
| ø Fast inward current density [pA/pF] ± SD | −686.1 ± 125.8 | 703.4 ± 55.3 | |
For illustrative purposes, an arbitrary threshold of 15% was defined and the differences in percentage of positive cells (% pos. cells) between beating and non-beating hiPSC-CMs > 15% are highlighted with a yellow shading. In the case of a significant difference between beating and non-beating hiPSC-CMs (* p < 0.05 and *** p < 0.001:), mean (ø) values are printed in blue. The covariance parameters were estimated using residual (restricted) maximum likelihood procedure (REML).