Table 3.

Results of single-cell RT-qPCR. Besides the percentage of positive cells (% pos. cells) and the number of investigated cells (n), also mean (ø) CT values and, mean (ø) ΔCT values are listed. ΔCT values are CT values normalized to the mean expression of TNNT2, and GAPDH of the respective cell group. For details see Methods section. Standard deviations (SD) are identical for CT and ΔCT values because the difference between the two values is a constant.

		HCN4	CACNA1G	CACNA1D	KCNA5	KCNJ4	SCN5A	KCNJ2	CACNA1C	KCNH2	TNNT2	GAPDH
iCell CMs	% pos. cells	100	40	29	29	65	100	31	98	98	100	98
	ø CT	27.94	29.88	31.56	32.05	30.99	27.44	31.77	27.43	27.34	20.38	24.06
	ø ΔCT	5.59	7.53	9.22	9.70	8.64	5.10	9.42	5.08	4.99
	± SD	1.49	1.89	1.56	1.46	1.29	1.61	1.26	1.81	1.47	1.02	1.17
	n	55	55	55	55	55	55	55	55	55	55	55
Ventr. PCs	% pos. cells	93	24	24	45	62	100	93	100	97	100	100
	ø CT	28.78	30.95	30.66	31.87	31.61	26.27	30.63	27.03	25.91	20.59	23.19
	ø ΔCT	6.89	9.06	8.77	9.99	9.72	4.39	8.74	5.14	4.02
	± SD	1.19	1.20	1.45	0.85	1.38	1.62	1.98	1.60	1.49	1.11	1.20
	n	29	29	29	29	29	29	29	29	29	29	29
Atr. PCs	% pos. cells	100	63	67	96	42	100	58	92	100	100	100
	ø CT	26.52	29.43	30.34	28.72	31.82	26.80	30.15	27.27	26.23	19.67	23.44
	mean ΔCT	4.97	7.87	8.79	7.17	10.27	5.25	8.59	5.71	4.68
	± SD	2.19	2.34	2.18	2.72	1.16	1.98	2.07	2.33	2.31	1.81	2.27
	n	24	24	24	24	24	24	24	24	24	24	24
Prim. ventr. CMs	% pos. cells	94	9	14	88	91	100	97	100	100	100	100
	ø CT	28.58	29.84	29.48	29.01	28.95	24.05	27.18	25.68	24.48	16.69	20.91
	ø ΔCT	9.78	11.04	10.68	10.21	10.16	5.25	8.38	6.88	5.68
	± SD	1.73	1.60	2.19	1.85	1.80	1.93	1.73	2.25	1.80	1.41	1.32
	n	66	66	66	66	66	66	66	66	66	66	66
Prim. atr. CMs	% pos. cells	95	53	65	100	95	100	80	98	100	100	100
	ø CT	28.11	30.35	30.43	27.11	29.72	24.85	29.98	25.95	25.75	17.93	21.25
	ø ΔCT	8.52	10.76	10.84	7.52	10.13	5.25	10.39	6.35	6.16
	± SD	2.02	1.39	1.84	1.85	1.73	1.44	1.93	1.63	1.67	1.37	1.32
	n	40	40	40	40	40	40	40	40	40	40	40