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. 2021 Dec 7;13(24):6156. doi: 10.3390/cancers13246156

Figure 2.

Figure 2

RT-PCR performed on frozen specimens of Pt#1 and Pt#3. (A) Relative expression of IL-6, IL-10, MMP14, and IL-1B is higher in wt rGBM than IDH1-mutant rGBM, indicating a prevalence of the M2-GAM subtype. YKL40, implicated in the regulation of GAM infiltration and migration, is upregulated in wt rGBM. IFNG expression is detected in wt rGBM, likely produced by CD8+ TILs. (B) Genes related to a terminal dysfunction of TILs are found in wt rGBM, where CD8+ TILs are abundant. PD-1, EOMES, BLIMP-1, and TOX indicate a terminal differentiation of infiltrating T cells. (C) CCL4 and CCL5 genes involved in TIL recruitment are upregulated in Pt#1. T-BET and GATA3 genes indicating the Th subtype show a prevalence of Th2 phenotype supporting an immunosuppressive microenvironment. (D) A molecular signature related to microglia is upregulated in IDH1-mutant rGBM, including P2RY12 and TMEM119, described as coexpressed in microglia.