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. 2021 Dec 6;10(12):3424. doi: 10.3390/cells10123424

Figure 4.

Figure 4

GEN ameliorated DEX-induced cholesterol accumulation by increasing ABCA1 expression. (A,B) The in vivo immunohistochemical examination of ABCA1 expression was conducted. (C–E) The protein expression of ABCA1 and ApoA-I was detected by western blot in DEX-treated MC3T3-E1 cells. (F) The level of the total intracellular cholesterol was measured using the ELISA kit in MC3T3-E1 cells co-treated with DEX and DIDS (200 μM). (G–I) The protein expression of ABCA1 and ApoA-I was detected by western blot in DEX/DIDS-treated MC3T3-E1 cells. (J) The ALP staining and the Alizarin Red S staining assays were conducted (×100 magnification). (K–M) The protein expression of RUNX2 and OPN was detected by western blot in DEX/DIDS-treated MC3T3-E1 cells. All experiments were implemented separately in triplicate. * p < 0.05; ** p < 0.01. OIM, osteogenic induction medium. NC, negative control; 50 mg/kg, Dex + 50 mg/kg GEN; 100 mg/kg, Dex + 100 mg/kg GEN.