Figure 3.

Optimizations for on-chip spheroid hanging drop culture. (A) Fluorescence images of FITC-containing droplets on hydrophobic and hydrophilic extruded circular island features. (B) Droplet height with different island diameter and volume of droplets on both hydrophobic and hydrophilic surfaces. Data was presented as mean ± SD. (n = 3) (C) Effect of cell number, droplet volumes, and island size on MCF-7 spheroid formation. Patterned islands consist of a microwell feature to facilitate spheroid centralization.