TABLE 1.
Effects of cisplatin on human melanoma cell lines
| Cell line | LD50a (μM) | Modulationb (mean ± SD)
|
|
|---|---|---|---|
| Bak | Bax | ||
| 224 | 20 | 2.7 ± 1.0 | 1.0 ± 0.3 |
| FM55 | 23 | 2.4 ± 1.4 | 1.4 ± 0.2 |
| AA | 40 | 3.5 ± 0.2 | 1.0 ± 0.1 |
| DFW | 60 | 2.3 ± 0.8 | 0.7 ± 0.5 |
a
Dose required to elicit 50% nuclear fragmentation after 24 h.
b
Induced by 20 μM (224 and FM55) or 40 μM cisplatin (AA and DFW), expressed as fold increase in median Bak- or Bax-associated immunofluorescence, using antibodies specific for an N-terminal epitope. All samples were prepared at 16 h after cisplatin addition.