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. 2021 Dec 7;10(12):3444. doi: 10.3390/cells10123444

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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HO-1 inducer enhances macrophage differentiation and HO-1 inhibitor suppresses the differentiation. Murine BMCs were treated with 20 μM hemin, 20 μM ZnPP, or 20 μM ZnPP with 100 μM CORM-3 on day 5 and incubated for 5 days to induce macrophage differentiation. (A) Cells were fixed and stained with antibodies F4/80-FITC and CD11b-PE. Cells were acquired on FACSCalibur, X axis: F4/80⁺, Y axis: CD11b⁺. (B) F4/80-positive and CD11b-positive cells were gated based on the isotype control. Results are presented as mean ± SD (n = 3 except n = 2 for CORM-3+ZnPP); ** p < 0.01 compared to control (one-way ANOVA).