Table 1.
TaqMan Gene Expression Assays used for relative quantitation real-time PCR.
| Protein | Gene | Gene Expression Assay ID |
|---|---|---|
| Acetyl-coenzyme A carboxylase alpha | Acca | Mm01304285_m1 |
| Acetyl-coenzyme A carboxylase beta | Accb | Mm01204683_m1 |
| Agouti related neuropeptide | Agrp | Mm00475829_g1 |
| Apolipoprotein B | Apob | Mm01545150_m1 |
| Beta-2-microglobulin | B2m | Mm00437762_m1 |
| Beta-actin | Actb | Mm00607939_s1 |
| Carnitine palmitoyltransferase 1a | Cpt1a | Mm01231183_m1 |
| Carnitine palmitoyltransferase 1b | Cpt1b | Mm00487191_g1 |
| CD68 antigen | Cd68 | Mm03047343_m1 |
| Chemokine (C-C motif) ligand 3 | Ccl3 | Mm00441259_g1 |
| Corticotropin releasing hormone | Crh | Mm01293920_s1 |
| Deiodinase, iodothyronine, type II | Dio2 | Mm00515664_m1 |
| Fatty acid synthase | Fasn | Mm00662319_m1 |
| Fibroblast growth factor 21 | Fgf21 | Mm00840165_g1 |
| Glucokinase | Gck | Mm00439129_m1 |
| Glucose-6-phosphatase, catalytic | G6pc | Mm00839363_m1 |
| Hypocretin | Hcrt | Mm01964030_s1 |
| Hypoxia inducible factor 1, alpha subunit | Hif1a | Mm00468869_m1 |
| Insulin receptor | Insr | Mm01211875_m1 |
| Insulin receptor substrate 1 | Irs1 | Mm01278327_m1 |
| Insulin receptor substrate 2 | Irs2 | Mm03038438_m1 |
| Insulin-like growth factor 1 | Igf1 | Mm00439560_m1 |
| Interleukin 1 beta | Il1b | Mm00434228_m1 |
| Klotho beta | Klb | Mm00473122_m1 |
| Leptin receptor | Lepr | Mm00440181_m1 |
| Lipase, hormone sensitive | Lipe | Mm00495359_m1 |
| Lipoprotein lipase | Lpl | Mm00434764_m1 |
| Melanocortin receptor type 4 | Mc4r | Mm00457483_s1 |
| Microsomal triglyceride transfer protein | Mttp | Mm00435015_m1 |
| Neuropeptide Y | Npy | Mm01410146_m1 |
| Nuclear factor, erythroid derived 2, like 2 | Nfe2l2 | Mm00477784_m1 |
| Patatin-like phospholipase domain containing 2 (adipose triglyceride lipase (ATGL)) | Pnpla2 | Mm00503040_m1 |
| Peroxisome proliferative activated receptor, gamma, coactivator 1 alpha |
Ppargc1a
(Pgc1) |
Mm01208835_m1 |
| Peroxisome proliferator activated receptor alpha | Ppara | Mm0040939_m1 |
| Peroxisome proliferator activated receptor gamma | Pparg | Mm00440940_m1 |
| Phosphoenolpyruvate carboxykinase 1, cytosolic | Pck1 | Mm01247058_m1 |
| Pro-opiomelanocortin | Pomc | Mm00435874_m1 |
| Pyruvate dehydrogenase kinase, isoenzyme 4 | Pdk4 | Mm01166879_m1 |
| Pyruvate kinase liver and red blood cell | Pklr | Mm00443090_m1 |
| Signal transducer and activator of transcription 5A | Stat5 | Mm03053818_s1 |
| Solute carrier family 2 (facilitated glucose transporter), member 2 (GLUT2) | Slc2a2 | Mm00446229_m1 |
| Solute carrier family 2 (facilitated glucose transporter), member 4 (GLUT4) | Slc2a4 | Mm00436615_m1 |
| Tissue inhibitor of metalloproteinase 1 | Timp1 | Mm01341361_m1 |
| Transforming growth factor, beta 1 | Tgfb1 | Mm01178820_m1 |
| Tumor necrosis factor alpha | Tnfa | Mm00443258_m1 |
| Uncoupling protein 1 (mitochondrial, proton carrier) | Ucp1 | Mm01244861_m1 |
| Uncoupling protein 3 (mitochondrial, proton carrier) | Ucp3 | Mm01163394_m1 |