TABLE 2.
Reactivity of colostra to EPEC antigens by ELISA and immunoblottinga
| Sample no. | BfpA
|
EspA
|
EspB
|
|||
|---|---|---|---|---|---|---|
| E | Ib | E | Ib | E | Ib | |
| 1 | − | − | 16 | + | 16 | + |
| 2 | − | + | − | + | 16 | + |
| 3 | 40 | + | − | + | − | − |
| 4 | 80 | − | − | + | − | + |
| 5 | 320 | + | − | − | − | + |
| 6 | 80 | + | 256 | + | 8 | + |
| 7 | − | − | − | − | − | − |
| 8 | 160 | + | 8 | + | − | − |
| 9 | 80 | + | 16 | + | 16 | + |
| 10 | 40 | − | 256 | + | − | − |
| 11 | 320 | + | 16 | + | − | − |
| 12 | − | − | − | + | − | − |
| 13 | 160 | + | − | − | 128 | + |
| 14 | − | − | 32 | + | − | − |
| 15 | − | + | 256 | + | 128 | + |
| 16 | − | − | 32 | + | 32 | + |
| 17 | − | + | − | − | − | − |
| 18 | − | − | 256 | + | 64 | + |
| 19 | 40 | + | 128 | − | 32 | + |
| 20 | − | − | 16 | + | − | − |
| 21 | − | − | 16 | + | 8 | + |
a
The numbers in columns E (ELISA) represent the titer of each sample expressed as the reciprocal of the dilution at which a positive reaction was observed (for BfpA, 1:10 to 1:20 = negative, 1:40 to 1:80 = weak positive, and >1:160 = positive; for EspA and EspB, 1:2 to 1:4 = negative, 1:8 to 1:16 = weak positive, and >1:32 = positive). Dilutions with absorbance values below 0.2 were considered negative. Ib, immunoblotting.