Figure 8.

Determination of the production of ROS using the 2’,7’-dichlorofluorescin diacetate (DCF-DA) probe. pbMECs were treated with LPS (10 μg/mL) or S. aureus (1 × 10⁷ particles/mL) for 6 and 12 h, followed by incubation with serum-free medium containing H2DCF-DA (20 µM) for 30 min. 2,7-dichlorofluorescein (DCF) fluorescence was measured using flow cytometry. (A) Representative flow cytometry histograms showing DCF-DA staining of cells treated with LPS or S. aureus for 12 h. (B) Mean fluorescence intensities (MIF) are shown as bar graph. Data are mean ± s.d. (n = 3). * p < 0.05.