Figure 4. Lin-, c-Kit+, Sca1+ (LSK) and hematopoietic stem cell (HSC) cluster-specific differential gene expression cannot be explained by receptor expression.

(A–D) Stacked bar graphs with proportion of differentially expressed genes that are unique for HSCs (purple), LSKs (green) or common (gray) upon granulocyte colony-stimulating factor (G-CSF) (A), poly(I:C) (B), Indo (C), or 16,16-dimethyl prostaglandin E₂ (dmPGE₂) (D) treatment. Below each bar graph the total number of differentially expressed genes (‘genes #’) for each fold-change (‘cutoff’) is listed. (E–F) Violin plots of receptor expression in control HSCs (E) and LSKs (F) split by cluster (only clusters with >20 cells displayed).