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. 2021 Dec 18;10(12):3581. doi: 10.3390/cells10123581

Figure 6.

Figure 6

Effects of neutrophil extracellular traps (NETs) and vesicles (nEVs) on platelets. Human platelets were stimulated with nEVs (A–C) or NETs (D–F) from control or PLY-stimulated neutrophils, and CD62P/annexin V expression was assessed by flow cytometry. (A) Percentage of CD62P positive platelets, (B) median fluorescence intensity (MFI) of CD62P in platelets, and (C) percentage of annexin V positive platelets treated with nEVs (derived from Ctr- or PLY-treated neutrophils; nEVCtr, nEVPLY). (D) Percentage of CD62P positive platelets, (E) MFI of CD62P in platelets, and (F) percentage of annexin V positive platelets treated with NETs (from Ctr- or PLY-treated neutrophils; NETCtr, NETPLY). n = 5–10. * p < 0.05, ** p < 0.01, *** p < 0.001, Kruskal–Wallis test.