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. 2021 Dec 16;10(12):3550. doi: 10.3390/cells10123550

Figure 3.

Figure 3

Influence of PXR activation on the PPARα-mediated reporter gene transcription under the control of the human HMGCS2 promoter. (A) HepG2 cells were transfected with a reporter construct including −6784 to +42 of human HMGCS2 in combination with or without a hPXR-expressing plasmid. The cells were treated with vehicle (0.2% DMSO), 100 µM bezafibrate (BZF), and/or 10 µM rifampicin (Rif) for 24 h, and reporter activity was determined. Data are shown as the mean ± S.D. (n = 4). (B) HepG2 cells were transfected with a hPXR expression plasmid and a reporter plasmid containing the −250 to +42 of human HMGCS2. The cells were then treated with vehicle (0.2% DMSO), 100 µM bezafibrate (BZF), and/or 10 µM rifampicin (Rif) for 24 h, and the reporter activity was determined. Closed and open circles in the plasmid diagrams represent the wild type and mutated DR1 motifs, respectively. Data are shown as the means ± S.D. (n = 4). (C) HepG2 cells were transfected with a hPPARα expression plasmid (10 ng) and/or hPXR expression plasmid (0.5, 5, or 50 ng) and a reporter plasmid containing the −250 to +42 of human HMGCS2. The cells were then treated with vehicle (0.2% DMSO), 100 µM bezafibrate (BZF), and/or 10 µM rifampicin for 24 h and the reporter activity was determined. Data are shown as the mean ± S.D. (n = 4).