Figure 4.

PXR-mediated suppression of PPARα-dependent reporter gene transcription. HepG2 cells were transfected with a reporter plasmid containing the −250 to +42 of human HMGCS2, expression plasmids for hPPARα, hPXR, and/or hPXR-ΔAF2 as indicated, and control phRL-SV40, followed by treatment with vehicle (0.2% DMSO), 100 μM bezafibrate (BZF), and/or 10 μM rifampicin (Rif) for 24 h. Relative reporter activity was determined. Data are shown as the mean ± S.D. (n = 4). * p < 0.05, Student’s t-test). The numbers above the bars indicate relative luciferase activity.